Gibco™ William's E Medium, GlutaMAX™ Supplement

Description

• William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells
• Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells
• This William's E Medium is modified as follows: With: Phenol Red, GlutaMAX™ Without: HEPES The complete formulation is available
• Gibco™ William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose
• William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate
• Gibco™ William's E Medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
• cGMP Manufacturing Gibco™ William's E Medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK
• The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
• William's E Medium contains no proteins or growth factors
• Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS)
• William's E Medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH.