Gibco™ LV-MAX™ Lentiviral Production System

Description

• The Gibco LV-MAX Lentiviral Production System is the first optimized system that provides a scalable and high-yield lentiviral vector production platform
• It is based on a suspension, high-density culture of HEK 293-derived Viral Production Cells adapted to a special chemically defined, serum-free and protein-free LV-MAX Production Medium
• Optimal viral titer yield is mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel lentiviral-specific enhancer and supplement
• All components work synergistically to generate superior and functional lentiviral particles under serum-free conditions compared to conventional polyethylenimine (PEI)
• The LV-MAX Lentiviral Production System provides: High titer—greater than 1 x 10 8 TU/mL (unconcentrated LVVs-GFP) Scalable suspension system—from 96 deep-well plate to 2-L bioreactor Serum-free—eliminating animal-origin components and associated risks Easy, robust culture and transfection protocols—fits into current transient production workflows The components included in the LV-MAX Lentiviral Production System are: two vials of frozen Viral Production Cells, one liter of LV-MAX Production Medium, and one LV-MAX Transfection Kit sufficient to produce 0.3 L of lentivirus
• All components of the LV-MAX Lentiviral Production System are also available for purchase separately
• Also required, but not included in this kit, is Gibco Opti-MEM I Reduced Serum Medium (Cat
• No
• 31985062).