Fmoc-Glu(OtBu)-Thr(psiMe,Mepro)-OH

Description

• General description: This pseudoproline dipeptide is undoubtedly the most effective and simplest to use tool for overcoming aggregation and enhancing peptide quality in Fmoc SPPS for peptide containing the Glu-Thr dipeptide motif. It consists of a dipeptide in which the threonine residue has been reversibly protected as a structure-breaking proline-like TFA-labile oxazolidine. The reason the pseudoproline residue is introduced as a dipeptide is because it avoids the need to acylate the hindered oxazolidine nitrogen. This has the added advantage of extending the peptide chain by two residues in one step. The threonine residue is regenerated during the normal TFA-mediated cleavage reaction. Associated Protocols and Technical Articles Cleavage and Deprotection Protocols for Fmoc SPPS Overcoming Aggregation in Fmoc SPPS
• Linkage: Replaces: 05-20-1122
• Analysis Note: Color (visual): white to slight yellow to beigeAppearance of substance (visual): powderIdentity (IR): passes testOptical rotation α 25/D (c=1 in methanol): -27.5 - -22.5 °Purity (TLC(157A)): ≥ 97 %Assay (HPLC, area%): ≥ 97.0 % (a/a)Impurities (single): ≤ 1.50 % (a/a)Acetate (IC): ≤ 0.1 %Ethyl acetate (HS-GC): ≤ 2.0 %To see the solvent systems used for TLC of Novabiochem® products please click here.
• Legal Information: Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany