Description

• General description: A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis.
• Application: The non-detergent sulfobetaine-201 acts as a pharmacological chaperone to promote folding and crystallization of the type II TGF-ß receptor extracellular domain.: This study explores the role of NDSB-201 in enhancing the folding and crystallization of a specific protein domain, highlighting its potential as a stabilizing agent in protein biochemistry (Kiessling et al., 2015). Stabilizing additives added during cell lysis aid in the solubilization of recombinant proteins.: Research demonstrated that NDSB-201, among other additives, assists in the solubilization of proteins during cell lysis, suggesting its effectiveness in improving recombinant protein yield (Van Voorhis et al., 2012).Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions.: Discusses the impact of various reagents, including NDSB-201, on protein refolding, providing insights into how these additives interact and influence protein structure restoration (Fox et al., 2005). Preparation of functional recombinant protein from E. coli using a nondetergent sulfobetaine.: Highlights the utility of NDSB-201 in preparing functional recombinant proteins from E. coli, underscoring its practical applications in molecular biology and biochemistry (Chen et al., 2000).
• Warning: Toxicity: Irritant (B)
• Reconstitution: Following reconstitution, store at room temperature. Stock solutions are stable for up to 3 months at room temperature.
• Other Notes: Goldberg, M.E., et al. 1996. Folding & Design 1, 21.Vuillard, L., et al. 1995. Biochem. J. 305, 337.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany