51558

HEPES buffered saline

Manufacturer: Sigma Aldrich

Synonym(S): HEPES Saline Buffer Solution

Select a Size

Pack Size SKU Availability Price
50 ML 51558-50-ML In Stock ₹ 5,867.15

51558 - 50 ML

₹ 5,867.15

In Stock

Quantity

1

Base Price: ₹ 5,867.15

GST (18%): ₹ 1,056.087

Total Price: ₹ 6,923.237

grade

for molecular biology

Quality Level

200

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L HEPES, 10 g/L KCl, 0.74 g/L NaCl, 16 g/L Na2HPO4.2H2O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected RNases, none detected phosphatases, none detected proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

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Description

  • General description: HEPES buffered saline is a tissue culture buffer,[1] which is used to maintain the pH of cell culture.[2] It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.[3]
  • Application: HEPES buffered saline has been used:for the preparation of plasmid DNA[4][5] as a reagent to prepare transfection master mix for DNA construct vectors[6]to develop hydrogel precursor for cell leakage assay[7]
  • Components: concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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grade:
for molecular biology

Quality Level:
200

product line:
BioUltra

form:
liquid

quality:
2× concentrate

composition:
dextrose, 2.0 g/L HEPES, 10 g/L KCl, 0.74 g/L NaCl, 16 g/L Na2HPO4.2H2O, 0.27 g/L

concentration:
2 ×

impurities:
DNases, none detected RNases, none detected phosphatases, none detected proteases, none detected

refractive index:
n20/D 1.339

pH:
7.2 (25 °C)

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