68268

Imidazole buffer Solution

Manufacturer: Sigma Aldrich

CAS Number: 288-32-4

Synonym(S): Imidazole solution, Glyoxaline solution

Select a Size

Pack Size SKU Availability Price
100 ML 68268-100-ML In Stock ₹ 9,774.98
500 ML 68268-500-ML In Stock ₹ 23,317.05

68268 - 100 ML

₹ 9,774.98

In Stock

Quantity

1

Base Price: ₹ 9,774.98

GST (18%): ₹ 1,759.496

Total Price: ₹ 11,534.476

product line

BioUltra

Quality Level

200

form

liquid

concentration

1 M in H2O

impurities

DNases, none detected RNases, none detected insoluble matter, passes filter test phosphatases, none detected proteases, none detected

pH

9.5±0.5

density

1.01 g/mL at 20 °C

SMILES string

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

Other Options

Image Product Name Manufacturer Price Range
NC1996852
U.S. Pharmacopeia Imidazole | 288-32-4 | MFCD00005183 | 200 mg
U.S. Pharmacopeia ₹ 88,006.76
50-242-4595
eMolecules​ Ambeed / 1H-Imidazole / 25g / 490548414 / A277098 / / 288-32-4 / MFCD00005183 / 68.079 / C3H4N2
eMolecules​ ₹ 2,878.26
50-188-3397
Sigma Aldrich Fine Chemicals Biosciences Imidazole | 288-32-4 | MFCD00005183 | 100g
Sigma Aldrich Fine Chemicals Biosciences ₹ 8,875.08
50-184-4763
Sigma Aldrich Fine Chemicals Biosciences Imidazole anhydrous, free-flowing, Redi-Dri(TM), ACS reagent, >=99% | 288-32-4 | MFCD00005183 | 100G
Sigma Aldrich Fine Chemicals Biosciences ₹ 7,022.10
50-184-4764
Sigma Aldrich Fine Chemicals Biosciences Imidazole anhydrous, free-flowing, Redi-Dri(TM), ACS reagent, >=99% | 288-32-4 | MFCD00005183 | 1KG
Sigma Aldrich Fine Chemicals Biosciences ₹ 31,944.77
50-184-4756
Sigma Aldrich Fine Chemicals Biosciences Imidazole BioUltra, >=99.5% (GC) | 288-32-4 | MFCD00005183 | 1KG
Sigma Aldrich Fine Chemicals Biosciences ₹ 2,96,539.10
50-184-4758
Sigma Aldrich Fine Chemicals Biosciences Imidazole BioUltra, >=99.5% (GC) | 288-32-4 | MFCD00005183 | 50G
Sigma Aldrich Fine Chemicals Biosciences ₹ 45,817.20
50-188-3399
Sigma Aldrich Fine Chemicals Biosciences Imidazole puriss. p.a., >=99.5% (GC) | 288-32-4 | MFCD00005183 | 500G
Sigma Aldrich Fine Chemicals Biosciences ₹ 21,368.90
50-184-4776
Sigma Aldrich Fine Chemicals Biosciences Imidazole ReagentPlus(R), 99% | 288-32-4 | MFCD00005183 | 500G
Sigma Aldrich Fine Chemicals Biosciences ₹ 13,519.10
50-491-515
Chem-Impex International, Inc. Imidazole | 288-32-4 | MFCD00005183 | 1KG
Chem-Impex International, Inc. ₹ 5,874.00

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Description

  • General description: Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.
  • Application: Imidazole buffer solution has been used as a component: of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteinsof lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assaysof imidazole wash and elution buffer for protein purification

SAFETY INFORMATION

Pictograms

GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H360D

Precautionary Statements

P201 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P313

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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form:
liquid

concentration:
1 M in H2O

impurities:
DNases, none detected RNases, none detected insoluble matter, passes filter test phosphatases, none detected proteases, none detected

pH:
9.5±0.5

density:
1.01 g/mL at 20 °C

SMILES string:
c1c[nH]cn1

InChI:
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key:
RAXXELZNTBOGNW-UHFFFAOYSA-N

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1S/2C12H27P.Pt/c2*1-10(2,3)13(11(4,5)6)12(7,8)9;/h2*1-9H3;

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RJQWVEJVXWLMRE-UHFFFAOYSA-N

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