Description

• General description: 18:0-20:4 DG or 1-stearoyl-2-arachidonoyl-sn-glycerol is a diglyceride.[1] Diglycerides contain two fatty acids linked to the glycerol backbone with an ester bond.[2]
• Application: 18:0-20:4 DG or 1-stearoyl-2-arachidonoyl-sn-glycerol has been used to study its effect on conventional protein kinase C (cPKC) and novel protein kinase C (nPKC) isozymes in vitro.[3] It has also been used as a substrate for the measurement of diacylglycerol kinase δ (DGK#948;)[4] and DGKη1 activity in vitro.[5]
• Biochem/physiol Actions: In biochemical signaling, diacylglycerol (DAG) functions as a second messenger signaling lipid, and is a product of the hydrolysis of the phospholipid PIP2 (phosphatidylinositolbisphosphate) by the enzyme phospholipase C (PLC) (a membrane-bound enzyme) that, through the same reaction, produces inositol trisphosphate (IP3). Although inositol trisphosphate (IP3) diffuses into the cytosol, DAG remains within the plasma membrane due to its hydrophobic properties. IP3 stimulates the release of calcium ions from the smooth endoplasmic reticulum, whereas DAG is a physiological activator of protein kinase C (PKC). The production of DAG in the membrane facilitates translocation of PKC from the cytosol to the plasma membrane.[6][7][8][9]Diacylglycerol mimicks the effects of the tumor-promoting compounds phorbol esters.
• Packaging: 30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (800818C-10mg)
• Storage and Stability: Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.
• Other Notes: Delivery to cells:Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.