Description

• General description: Spermidine trihydrochloride is an aliphatic polyamine. Its crystalline structure has been studied.[1] It has been reported to preserve the nuclei and prevent proteolytic degradation of histones during the macronuclei isolation from Tetrahymena pyriformis.[2]
• Application: Spermidine trihydrochloride is used for the following applications: DNA extraction and restriction endonuclease digestion[3]Nuclear transplantation[4]During the chemical analyses of the squid species Todaropsis eblanae[5]As one of the agents in intercalating drug.[6]Platelet aggregation[7]It may be used in the synthesis of palladium complexes like [sperH3]2[PdCl4]3, [PdCl2(sperH)]2[PdCl4], and [(PdCl2)3(sper)2] by reacting with K2PdCl4 and PdCl2.[8]
• Biochem/physiol Actions: Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
• Caution: Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.
• Other Notes: Buffer component for chromosome isolation in bivariate flow cytogenetic analysis and sorting[9].