93321
TRIS Glycine buffer solution
Manufacturer: Sigma Aldrich
Synonym(S): Blotting buffer solution
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 L | 93321-1-L | In Stock | ₹ 10,458.23 |
93321 - 1 L
In Stock
Quantity
1
Base Price: ₹ 10,458.23
GST (18%): ₹ 1,882.481
Total Price: ₹ 12,340.711
product line
BioUltra
Quality Level
100
form
liquid
quality
10× concentrate
composition
glycine, 1.92 M TRIS, 0.25 M
impurities
insoluble matter, passes filter test proteases, none detected
ign. residue
≤0.2%
pH
8.5-8.7
anion traces
sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kgAs: ≤0.1 mg/kgBa: ≤5 mg/kgBi: ≤5 mg/kgCa: ≤10 mg/kgCd: ≤5 mg/kgCo: ≤5 mg/kgCr: ≤5 mg/kgCu: ≤5 mg/kgFe: ≤5 mg/kgK: ≤50 mg/kgLi: ≤5 mg/kgMg: ≤5 mg/kgMn: ≤5 mg/kgMo: ≤5 mg/kgNH4+: ≤200 mg/kgNa: ≤500 mg/kgNi: ≤5 mg/kgPb: ≤5 mg/kgSr: ≤5 mg/kgZn: ≤5 mg/kg
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Description
- Application: Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.
- Other Notes: Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications[1]
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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product line: BioUltra
Quality Level: 100
form: liquid
quality: 10× concentrate
composition: glycine, 1.92 M TRIS, 0.25 M
impurities: insoluble matter, passes filter test proteases, none detected
ign. residue: ≤0.2%
pH: 8.5-8.7
anion traces: sulfate (SO42-): ≤50 mg/kg
cation traces: Al: ≤5 mg/kgAs: ≤0.1 mg/kgBa: ≤5 mg/kgBi: ≤5 mg/kgCa: ≤10 mg/kgCd: ≤5 mg/kgCo: ≤5 mg/kgCr: ≤5 mg/kgCu: ≤5 mg/kgFe: ≤5 mg/kgK: ≤50 mg/kgLi: ≤5 mg/kgMg: ≤5 mg/kgMn: ≤5 mg/kgMo: ≤5 mg/kgNH4+: ≤200 mg/kgNa: ≤500 mg/kgNi: ≤5 mg/kgPb: ≤5 mg/kgSr: ≤5 mg/kgZn: ≤5 mg/kg
product line:
BioUltra
Quality Level:
100
form:
liquid
quality:
10× concentrate
composition:
glycine, 1.92 M TRIS, 0.25 M
impurities:
insoluble matter, passes filter test proteases, none detected
ign. residue:
≤0.2%
pH:
8.5-8.7
anion traces:
sulfate (SO42-): ≤50 mg/kg
cation traces:
Al: ≤5 mg/kgAs: ≤0.1 mg/kgBa: ≤5 mg/kgBi: ≤5 mg/kgCa: ≤10 mg/kgCd: ≤5 mg/kgCo: ≤5 mg/kgCr: ≤5 mg/kgCu: ≤5 mg/kgFe: ≤5 mg/kgK: ≤50 mg/kgLi: ≤5 mg/kgMg: ≤5 mg/kgMn: ≤5 mg/kgMo: ≤5 mg/kgNH4+: ≤200 mg/kgNa: ≤500 mg/kgNi: ≤5 mg/kgPb: ≤5 mg/kgSr: ≤5 mg/kgZn: ≤5 mg/kg
product line: BioUltra
Quality Level: 100
form: powder or crystals
quality: __
composition: __
impurities: insoluble matter, passes filter test
ign. residue: __
pH: 3.0-4.5 (25 °C, 0.5 M in H2O)
anion traces: chloride (Cl-): ≤50 mg/kgsulfate (SO42-): ≤50 mg/kg
cation traces: Al: ≤5 mg/kgAs: ≤0.1 mg/kgBa: ≤5 mg/kgBi: ≤5 mg/kgCa: ≤10 mg/kgCd: ≤5 mg/kgCo: ≤5 mg/kgCr: ≤5 mg/kgCu: ≤5 mg/kgFe: ≤5 mg/kgK: ≤50 mg/kgLi: ≤5 mg/kgMg: ≤5 mg/kgMn: ≤5 mg/kgMo: ≤5 mg/kgNa: ≤50 mg/kgNi: ≤5 mg/kgPb: ≤5 mg/kgSr: ≤5 mg/kgZn: ≤5 mg/kg
product line:
BioUltra
Quality Level:
100
form:
powder or crystals
quality:
__
composition:
__
impurities:
insoluble matter, passes filter test
ign. residue:
__
pH:
3.0-4.5 (25 °C, 0.5 M in H2O)
anion traces:
chloride (Cl-): ≤50 mg/kgsulfate (SO42-): ≤50 mg/kg
cation traces:
Al: ≤5 mg/kgAs: ≤0.1 mg/kgBa: ≤5 mg/kgBi: ≤5 mg/kgCa: ≤10 mg/kgCd: ≤5 mg/kgCo: ≤5 mg/kgCr: ≤5 mg/kgCu: ≤5 mg/kgFe: ≤5 mg/kgK: ≤50 mg/kgLi: ≤5 mg/kgMg: ≤5 mg/kgMn: ≤5 mg/kgMo: ≤5 mg/kgNa: ≤50 mg/kgNi: ≤5 mg/kgPb: ≤5 mg/kgSr: ≤5 mg/kgZn: ≤5 mg/kg
product line: __
Quality Level: 100
form: powder
quality: __
composition: __
impurities: __
ign. residue: __
pH: __
anion traces: __
cation traces: __
product line:
__
Quality Level:
100
form:
powder
quality:
__
composition:
__
impurities:
__
ign. residue:
__
pH:
__
anion traces:
__
cation traces:
__
product line: BioUltra
Quality Level: 300
form: crystalline powder
quality: __
composition: __
impurities: insoluble matter, passes filter test
ign. residue: ≤0.2%
pH: 6.0-7.0 (25 °C, 0.5 M in H2O)
anion traces: chloride (Cl-): ≤50 mg/kgsulfate (SO42-): ≤50 mg/kg
cation traces: __
product line:
BioUltra
Quality Level:
300
form:
crystalline powder
quality:
__
composition:
__
impurities:
insoluble matter, passes filter test
ign. residue:
≤0.2%
pH:
6.0-7.0 (25 °C, 0.5 M in H2O)
anion traces:
chloride (Cl-): ≤50 mg/kgsulfate (SO42-): ≤50 mg/kg
cation traces:
__