Description

• General description: Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain.[1]
• Application: Amphotericin B solution has been used:as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice[2] as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries as a constituent of transport medium during the collection of equine corpora lutea[3] as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line[4]
• Biochem/physiol Actions: Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections.[5] It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.
• Packaging: 20,50,100ml
• Components: This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate.
• Caution: Amphotericin B remains active for 3 days in culture at 37°C.
• Preparation Note: It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.
• Other Notes: Keep container tightly closed in a dry and well-ventilated place. Light sensitive.