Description

• General description: Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. and has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes.
• Application: Amphotericin B is used to study the affects of the influx of calcium on antifungal activity, [1] and to study the binding and kinetics of Amphotericin B to sterols in monolayers, aqueous solutions, and phospholipid bilayers [2][3]. It is also commonly used in tissue culture as an antifungal supplement.Amphotericin B from Streptomyces sp. has been used-for amphotericin perforated patch technique[4]as a reference antifungal drug during MIC (minimum inhibitory concentration) agar dilution assay[5][6][7]as a component of Arcobacter selective isolation broth[8]
• Biochem/physiol Actions: Mode of Action: Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out.Antimicrobial spectrum: Includes fungi and yeast
• Features and Benefits: High quality antibiotic suitable for mulitple research applications
• Preparation Note: Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility.Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.
• Storage and Stability: Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible
• Other Notes: For additional information on our range of Biochemicals, please complete this form.