Acetyl-Coenzyme A

Description

• General description: Acetyl-Coenzyme A, trilithium salt
• Application: Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts.[1] Acetyl-Coenzyme A has also been used to determine citrate synthase activity.[2]
• Biochem/physiol Actions: Acetyl-Coenzyme A (Ac-CoA) is the end product of glycolysis and takes part in the Ac-CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis.[3][4][5][6] It also takes part in fatty acid biosynthesis and catabolism of polyamines like spermine and spermidine. A low level of Ac-CoA leads to a loss in glial cell and neuronal function. Ketone bodies and triglycerides give rise to Ac-CoA on hydrolysis and this indirectly leads to increased histone acetylation.[7][8][9]
• Preparation Note: Working solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).Storage conditions (working solution): -15 to -25 °C50 mg/ml in phosphate buffer, pH 7, is stable for 3 weeks at -15 to -25 °C. Unfrozen solutions should be immediately used.
• Other Notes: For life science research only. Not for use in diagnostic procedures.