Citrate Buffer Solution, 0.09 M

Description

• General description: Citrate Buffer Solution, 0.09 M, is a high-quality biochemical buffer solution commonly employed in various research applications. Its applications include use in acid phosphatase reactions alongside p-nitrophenyl phosphate (pNPP) enzyme substrate. Citrate Buffer is also used for the isolation of RNA from cells and tissues.[1] In addition, it is also used as a background electrolyte for separating sulfonamides utilizing capillary zone electrophoresis.[2] With its superior buffering capacity and ability to maintain a stable pH, Citrate Buffer Solution, 0.09 M is an excellent choice for any researcher in need of reliable performance.
• Application: Citrate Buffer Solution, 0.09 M has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections during immunohistochemistry (IHC) experiments.[3][4] It has also been used in the preparation of hybridization solutions for fluorescence in situ hybridization (FISH) procedures.[5]
• Other Notes: Unit definition: One unit of acid phosphatase will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per, minute at pH 4.8 at 37 °C.