Description

• General description: Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.
• Application: Deoxyribonucleic acid, single stranded from salmon testes has been used for/as:in situ hybridization histochemistry of rat brain[1], human kidney sections[2] and renal biopsy tissues[3] preparing standard curve of DNA[4] prehybridization solution in southern and northern hybridizations[5] dilution of the cDNA[6]fluorescent in-situ hybridization (FISH)[7]ChIP analysis[8]a standard to analyse the degraded nucleosides of genomic DNA by high performance liquid chromatography[9]a component of hybridization solution in FISH[10]a control in DNA-DNA hybridization experiment between various bacterial species[11]to precipitate probes in fluorescence in situ hybridization (FISH)[12][13][14]
• Preparation Note: This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
• Other Notes: DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.