DMT-2′O-Methyl-rG(ib) Phosphoramidite
configured for (ÄKTA® and OligoPilot®)
Manufacturer: Sigma Aldrich
CAS Number: 150780-67-9
Synonym(S): DMT-2′-O-Me-rG(ib) amidite, N-(2-methyl-1-oxopropyl)-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-guanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 G | G211150-5-G | In Stock | ₹ 48,853.23 |
| 10 G | G211150-10-G | In Stock | ₹ 98,128.63 |
G211150 - 5 G
In Stock
Quantity
1
Base Price: ₹ 48,853.23
GST (18%): ₹ 8,793.581
Total Price: ₹ 57,646.811
biological source
non-animal source (no BSE/TSE risk)
Quality Level
300
product line
Proligo Reagents
Assay
≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form
powder
technique(s)
oligo synthesis: suitable
impurities
≤0.1% single unspecified Impurity (reversed phase HPLC)≤0.3% mG2 (reversed phase HPLC, Hydrolysate)≤0.3% mG3 (reversed phase HPLC, DMT-rG(ib)me)≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤1.0% mG1 (reversed phase HPLC, DMT-rG(ib)me-DMT)≤3% residual Solvent content
color
white to off-white
λ
conforms (UV/VIS Identity)
suitability
conforms to structure for H-NMRconforms to structure for LC-MS
Other Options
Description
- General description: Proligo′s 2′O-Methyl RNA monomers are compatible with fast deprotectionschemes that are based on the application of aliphatic amines, such asmethylamine. The adenosine and guanosine monomers are protected bythe standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl RNA is a nucleic acid analog that is characterized by theexceptional hybridization properties that it imparts with complimentaryDNA or RNA, as well as increased stability against enzymatic degradationcompared to natural nucleic acids.The unique combination of properties of 2′O-Methyl RNA had foundwidespread use in the fields of:Diagnostic probesAptamer and ribozyme developmentMixed 2′O-Methyl-RNA/DNA antisense moleculesKey Features:High yield of crude oligonucleotidesCompatible with DNA synthesisCan be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotidesRecommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °CPurification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradationDMT-2′O-Methyl-rG(ib) Phosphoramidite is configured for ÄKTA® Synthesizers.
- Legal Information: OligoPilot is a registered trademark of Cytiva
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable