Description

• General description: Proligo′s 2′O-Methyl RNA monomers are compatible with fast deprotectionschemes that are based on the application of aliphatic amines, such asmethylamine. The adenosine and guanosine monomers are protected bythe standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl RNA is a nucleic acid analog that is characterized by theexceptional hybridization properties that it imparts with complimentaryDNA or RNA, as well as increased stability against enzymatic degradationcompared to natural nucleic acids.The unique combination of properties of 2′O-Methyl RNA had foundwidespread use in the fields of:Diagnostic probesAptamer and ribozyme developmentMixed 2′O-Methyl-RNA/DNA antisense moleculesKey Features:High yield of crude oligonucleotidesCompatible with DNA synthesisCan be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotidesRecommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °CPurification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradationDMT-2′O-Methyl-rG(ib) Phosphoramidite is configured for ÄKTA® Synthesizers.
• Legal Information: OligoPilot is a registered trademark of Cytiva