Description

• General description: HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]
• Application: HEPES has been used:As a component of homogenisation buffer used in protein sample preparation[3]To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation[4]As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation[5]As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.[6][7]
• Other Notes: Easily compare specifications for HEPES products with the HEPES specification table.