Description

• General description: Isopropyl β-D-Thiogalactopyranoside (IPTG) is a molecular biology reagent used to induce gene expression in bacterial systems. It mimics lactose, binding to the lac repressor protein, leading to gene activation. IPTG is crucial for studying gene regulation, protein expression, and recombinant protein production, providing valuable insights into cellular and molecular biology research.
• Application: IPTG has been used: in the induction of protein expression in Lactobacillus reuteri GroEL and mutants[1] in the induction of protein expression in Escherichia coli[2]
• Biochem/physiol Actions: IPTG is a non-metabolizable analog of allolactose. It acts as an inducer that regulates the transcriptional activity of the lac promoter, a promoter that is used for the expression of the protein in E.coli.[3]
• Other Notes: Non-metabolizable galactose analog.
• Reconstitution: A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.