Nitrite Assay Kit (Griess Reagent)

Description

• General description: Nitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and can also be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxiadependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration.
• Application: Convenient environmentally friendly on-site quantitative analysis of nitrite and nitrate in seawater based on polymeric test kits and smartphone application.: This research develops an eco-friendly and portable method for nitrite and nitrate analysis in seawater, employing polymeric test kits and a smartphone application for data analysis, facilitated by the Griess reagent for nitrite detection. (Choodum et al., 2020).MiR-124 inhibits spinal neuronal apoptosis through binding to GCH1.: The study investigates the role of miR-124 in preventing spinal neuronal apoptosis, utilizing a nitrite assay kit to measure nitric oxide levels as part of the biochemical analysis. (Yuan et al., 2019).Humoral factors in ALS patients during disease progression.: This paper examines the changes in humoral factors in ALS patients, measuring nitric oxide levels using a nitrite assay kit to assess inflammation and oxidative stress. (Ehrhart et al., 2015).Inducible nitric oxide synthase-nitric oxide plays an important role in acute and severe hypoxic injury to pancreatic beta cells.: The research highlights the significance of nitric oxide in hypoxic injury to pancreatic beta cells, using a nitrite assay kit to quantify nitric oxide levels. (Ko et al., 2008).
• Suitability: The kit is suitable for the detection of Nitric Oxide in animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma and urine.
• Principle: The Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at A540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.