MAK380

LDH-Cytotoxicity Assay Kit II

sufficient for 500 colorimetric tests

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
1 KIT MAK380-1-KIT In Stock ₹ 51,470.70

MAK380 - 1 KIT

₹ 51,470.70

In Stock

Quantity

1

Base Price: ₹ 51,470.70

GST (18%): ₹ 9,264.726

Total Price: ₹ 60,735.426

detection method

colorimetric

storage temp.

−20°C

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Description

  • General description: The new LDH Cytotoxicity Assay Kit, MAK529, is now available! Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.
  • Features and Benefits: The LDH-Cytotoxicity Assay Kit II:Is a non-radioactive assay.Simple one-step procedure.
  • Suitability: The kit is suitable for the determination of Lactate dehydrogenase (LDH) activity in cells.
  • Principle: The LDH Cytotoxicity Assay Kit II utilizes the advanced WST (Water Soluble Tetrazolium) reagent for a fast and more sensitive detection of LDH released from the damaged cells. The assay utilizes an enzymatic coupling reaction: LDH oxidizes lactate to generate NADH, which then reacts with WST to generate yellow color at A450 nm. The intensity of the generated color correlates directly with the cell number lysed.

SAFETY INFORMATION

Pictograms

GHS05

Signal Word

Danger

Hazard Statements

H314

Precautionary Statements

P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

WGK

WGK 3

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