Kinase Assay Kit

Description

• General description: Kinases, alternatively referred to as phosphotransferases, form an extensive family of enzymes responsible for transferring phosphate groups from the high-energy donor molecule ATP to their designated substrates. This family of enzymes is renowned for its role in regulating a myriad of cellular processes, with the protein kinases constituting the largest subgroup. In humans, 518 distinct kinases have been identified, and a substantial portion of human proteins, up to 30%, undergo modification by these kinases. The considerable diversity and pivotal role of kinases in cellular signaling position them as promising targets for drug development, biochemical and biomedical research.
• Application: The Kinase Assay Kit may be used for:Cancer ResearchCell Signaling ResearchDrug Discovery
• Features and Benefits: High Sensitivity and Accuracy: Achieve quantification of kinase activity, detecting as low as 0.01 U/L with high sensitivity and accuracy.Streamlined Procedure: Effortlessly conduct the assay with a simplified process, adding just a single working reagent. Complete the assay in a quick 10-minute reaction, optimizing efficiency and saving valuable time.High-Throughput Inhibitor Screening: Screen for kinase inhibitors effectively with a minimal 5 μL test compound requirement, enhancing efficiency in high-throughput settings.
• Suitability: The kit is suitable for the determination of protein kinase activity and for high-throughput screening of kinase inhibitors.
• Principle: The Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorometric (Ex = λ530 nm/λEm = 590 nm) assay method.
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