NAD+/NADH Assay Kit

Description

• General description: Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.
• Application: Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study.: This study investigates the role of glycolysis in the myometrium of patients with adenomyosis, utilizing the NAD+/NADH Assay Kit to assess metabolic alterations (Huang et al., 2024). 10.1080/09513590.2024.2332411Jian-Pi-Yi-Shen formula alleviates renal fibrosis by restoring NAD+ biosynthesis in vivo and in vitro.: This research demonstrates how the Jian-Pi-Yi-Shen formula mitigates renal fibrosis through NAD+ biosynthesis, with the NAD+/NADH Assay Kit playing a crucial role in the biochemical analysis (Gao et al., 2023). 10.18632/aging.205352Butyrate inhibits the mitochondrial complex Ι to mediate mitochondria-dependent apoptosis of cervical cancer cells.: The study explores butyrate′s effects on mitochondrial function and apoptosis in cervical cancer cells, employing the NAD+/NADH Assay Kit to measure mitochondrial activity (Zhang et al., 2023). 10.1186/s12906-023-04043-3PM2.5 mediates mouse testis Sertoli TM4 cell damage by reducing cellular NAD().: This article investigates the impact of PM2.5 on Sertoli cells in mouse testes, using the NAD+/NADH Assay Kit to track changes in cellular NAD levels (Xu et al., 2023). 10.1080/15376516.2023.2215862Synergistic Effect and Mechanism of Plumbagin with Gentamicin Against Carbapenem-Resistant Klebsiella pneumoniae.: The research highlights the synergistic antibacterial effects of plumbagin and gentamicin, with the NAD+/NADH Assay Kit being used to evaluate the metabolic impact on bacterial cells (Chen et al., 2020). 10.2147/IDR.S265753
• Features and Benefits: Broad Detection Range: Detect NAD and NADH levels accurately across a wide range, from 0.02 to 1 μM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirementSimplified Process: Experience a streamlined process with the addition of only a single working reagent and a 10 minute room temperature reaction, reducing complexity and saving valuable time and effort.Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
• Suitability: The kit is suitable for the determination of NAD and NADH and the evaluation of drug effects on NAD/NADH metabolism in cells, tissue extracts etc.
• Principle: The NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.
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