Description

• General description: Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]
• Application: TWEEN 20 has been used as a component of:washing buffer and blocking buffer in Western blotting[2]blocking buffer in immunohistochemistry[3]washing buffer in ELISA (enzyme linked immunosorbent assay)[4][5]reaction mixture in PCR (polymerase chain reaction)[6]
• Features and Benefits: Highly versatile surfactant for your life science and biochemical researchExcellent solubilising, dispersing and emulsifying agentSuitable for DNA & RNA extraction, mammalian cell culture, and protein purification
• Packaging: P1379-1KT:Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
• Preparation Note: TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: TWEEN is a registered trademark of Croda International PLC