Description

• General description: Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.
• Application: Sodium bicarbonate has been used:as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1]as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells[2]as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells[3]as a component of medium used for cell culture of human embryonic kidney cells[4]as a component of Beltsville thawing solution used for the dilution of semen[5]