S8140

SSPE Buffer

for Northern and Southern blotting, powder blend

Manufacturer: Sigma Aldrich

Synonym(S): Saline-Sodium Phosphate-EDTA Buffer

Select a Size

Pack Size SKU Availability Price
100 ML S8140-100-ML In Stock ₹ 1,931.40

S8140 - 100 ML

₹ 1,931.40

In Stock

Quantity

1

Base Price: ₹ 1,931.40

GST (18%): ₹ 347.652

Total Price: ₹ 2,279.052

grade

for molecular biology

Quality Level

200

form

powder blend

pH

7.2-7.6 (20 × in water, high purity, ambient)

solubility

water, high purity: 20 ×, clear, colorless

foreign activity

DNAse, none detectedEndonuclease, none detectedExonuclease, none detectedNICKase, none detectedRNAse, none detected

storage temp.

room temp

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Description

  • General description: SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.
  • Application: Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).
  • Features and Benefits: Easy to reconstitute powderMulti-purpose use in hybridizations
  • Components: Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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powder blend

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water, high purity: 20 ×, clear, colorless

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DNAse, none detectedEndonuclease, none detectedExonuclease, none detectedNICKase, none detectedRNAse, none detected

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