Rhodamine B labeled Polymyxin B Ready Made Solution

Description

• General description: Rhodamine B labeled polymyxin B (SRB-PMB) is a fluorescent derivative of Polymyxin B. Rhodamine B polymyxin B is an additional fluorescent derivative of Polymyxin B, along with Dansyl labeled polymyxin B (Product # SBR00029). Both products′ mode of action is in accordance with Polymyxin B activity. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.
• Application: Fluorescent antibiotics can be used for many applications[1] including:Antimicrobial resistance research.Bacterial visualization and imaging.Parent antibiotic mode of action research and new antibiotic discovery.Toxicity studies.Research of bacterial infections and tracking its uptake in vivo.Additional possible applications for Rhodamine B labeled Polymyxin B:Evaluate LPS binding by FRET (fluorescence resonance energy transfer) studies to form a specific complex between fluorescein (FITC)-tagged LPS and Polymyxin B, indicating direct binding of the antibiotic to the LPSCan be applied as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics to assess the mitochondrial function of polymyxin B in a kidney cell line (LLC-PK1), suggesting that it changes the mitochondrial membrane polarization.[2]
• Analysis Note: Rhodamine B labeled polymyxin B Ready Made Solution is light sensitive.It is recommended to avoid freeze-thaw cycles of Rhodamine B labeled polymyxin B Ready-Made SolutionRhodamine B labeled polymyxin B Ready Made Solution (1.1mg/mL) can be diluted 1:100-200 in PBSX1 (Product# D8537) to achieve 0.55-1.1μg/mL final concentration for staining. The above concentration of Rhodamine B labeled polymyxin B was used for Escherichia coli staining (see image) Fluorescence Microscopy application: Rhodamine B labeled polymyxin B Ready Made Solution excitation (Ex) wavelength is 550-560nm resulting in emission (Em) range of 580-610nm (λmax=590-600nm)