U211150
DMT-2′O-Methyl-rU Phosphoramidite
configured for ÄKTA® and OligoPilot®
Manufacturer: Sigma Aldrich
CAS Number: 110764-79-9
Synonym(S): 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-uridine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], DMT-2′O-Methyl-rU amidite
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 2 G | U211150-2-G | In Stock | ₹ 19,203.55 |
| 5 G | U211150-5-G | In Stock | ₹ 48,853.23 |
| 10 G | U211150-10-G | In Stock | ₹ 97,630.68 |
U211150 - 2 G
In Stock
Quantity
1
Base Price: ₹ 19,203.55
GST (18%): ₹ 3,456.639
Total Price: ₹ 22,660.189
biological source
non-animal source (no BSE/TSE risk)
Quality Level
300
product line
Proligo Reagents
Assay
≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form
powder
technique(s)
oligo synthesis: suitable
impurities
≤0.1% single unspecified Impurity (reversed phase HPLC)≤0.3% mU2 (reversed phase HPLC, Hydrolysate)≤0.3% mU3 (reversed phase HPLC, DMT-rme)≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT)≤3% residual Solvent content
color
white to off-white
λ
conforms (UV/VIS Identity)
suitability
conforms to structure for H-NMRconforms to structure for LC-MS
Other Options
Description
- General description: DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites.
- Application: 2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.
- Features and Benefits: Its key features include: High yield of crude oligonucleotides Compatible with DNA synthesis Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA (concentrated ammonia/ 40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers) 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry
- Other Notes: The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of: Diagnostic probes Aptamer and ribozyme development Mixed 2′O-Methyl-RNA/DNA antisense molecules
- Legal Information: OligoPilot is a registered trademark of Cytiva
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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biological source: non-animal source (no BSE/TSE risk)
Quality Level: 300
product line: Proligo Reagents
Assay: ≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form: powder
technique(s): oligo synthesis: suitable
impurities: ≤0.1% single unspecified Impurity (reversed phase HPLC)≤0.3% mU2 (reversed phase HPLC, Hydrolysate)≤0.3% mU3 (reversed phase HPLC, DMT-rme)≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT)≤3% residual Solvent content
color: white to off-white
λ: conforms (UV/VIS Identity)
suitability: conforms to structure for H-NMRconforms to structure for LC-MS
biological source:
non-animal source (no BSE/TSE risk)
Quality Level:
300
product line:
Proligo Reagents
Assay:
≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form:
powder
technique(s):
oligo synthesis: suitable
impurities:
≤0.1% single unspecified Impurity (reversed phase HPLC)≤0.3% mU2 (reversed phase HPLC, Hydrolysate)≤0.3% mU3 (reversed phase HPLC, DMT-rme)≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT)≤3% residual Solvent content
color:
white to off-white
λ:
conforms (UV/VIS Identity)
suitability:
conforms to structure for H-NMRconforms to structure for LC-MS
biological source: non-animal source (no BSE/TSE risk)
Quality Level: 300
product line: Proligo Reagents
Assay: ≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form: powder
technique(s): oligo synthesis: suitable
impurities: ≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤0.5% single Impurity (reversed phase HPLC)≤3% residual Solvent content
color: white to off-white
λ: conforms (UV/VIS Identity)
suitability: __
biological source:
non-animal source (no BSE/TSE risk)
Quality Level:
300
product line:
Proligo Reagents
Assay:
≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form:
powder
technique(s):
oligo synthesis: suitable
impurities:
≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤0.5% single Impurity (reversed phase HPLC)≤3% residual Solvent content
color:
white to off-white
λ:
conforms (UV/VIS Identity)
suitability:
__
biological source: non-animal source (no BSE/TSE risk)
Quality Level: 300
product line: Proligo Reagents
Assay: ≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form: powder
technique(s): oligo synthesis: suitable
impurities: ≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤0.5% single Impurity (reversed phase HPLC)≤3% residual Solvent content
color: white to off-white
λ: conforms (UV/VIS Identity)
suitability: conforms to structure for H-NMRconforms to structure for LC-MS
biological source:
non-animal source (no BSE/TSE risk)
Quality Level:
300
product line:
Proligo Reagents
Assay:
≥99% (31P-NMR)≥99.0% (reversed phase HPLC)
form:
powder
technique(s):
oligo synthesis: suitable
impurities:
≤0.3% water content (Karl Fischer)≤0.5% P(III) Impurities 100-169ppm (31P-NMR)≤0.5% single Impurity (reversed phase HPLC)≤3% residual Solvent content
color:
white to off-white
λ:
conforms (UV/VIS Identity)
suitability:
conforms to structure for H-NMRconforms to structure for LC-MS
biological source: __
Quality Level: 200
product line: Proligo Reagents
Assay: ≥98% (31P-NMR)≥98.0% (reversed phase HPLC)
form: solid
technique(s): oligo synthesis: suitable
impurities: ≤0.3 wt. % water content (Karl Fischer)≤0.5% 3′-TC isomer (reversed phase HPLC)≤0.5% P(III) Impurities 145-155ppm (31P-NMR)
color: white to light yellow
λ: conforms (UV/VIS Identity)
suitability: __
biological source:
__
Quality Level:
200
product line:
Proligo Reagents
Assay:
≥98% (31P-NMR)≥98.0% (reversed phase HPLC)
form:
solid
technique(s):
oligo synthesis: suitable
impurities:
≤0.3 wt. % water content (Karl Fischer)≤0.5% 3′-TC isomer (reversed phase HPLC)≤0.5% P(III) Impurities 145-155ppm (31P-NMR)
color:
white to light yellow
λ:
conforms (UV/VIS Identity)
suitability:
__