DIG Easy Hyb™ Granules

Description

• General description: DIG Easy Hyb™ is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
• Application: DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes.[1][2][3] It is specifically designed for use with DIG kits.
• Features and Benefits: DIG Easy Hyb™ is a convenient ready-to-use hybridization buffer, saving time and effort. DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.
• Preparation Note: Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
• Other Notes: For life science research only. Not for use in diagnostic procedures.
• Legal Information: DIG Easy Hyb is a trademark of Roche