Bilirubin Oxidase from Myrothecium verrucaria

Description

• General description: We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.Bilirubin oxidase (BOD) belongs to the subclass of the multicopper oxidase family.[1][2] It is usually found in fungi.[2]Bilirubin Oxidase is composed of three cupredoxin-like domains that result in two active sites formed by four copper ions.[3] Research area: Apoptosis
• Application: Bilirubin Oxidase from Myrothecium verrucaria has been used to eliminate the interference of bilirubin.[4] It has also been used to chemically modify with fluorescein derivative (FS) and combined with multivariate calibration to analyse direct bilirubin (dBR) and total bilirubin (tBR).[5]
• Biochem/physiol Actions: Bilirubin oxidase (BOD) is a multicopper oxidase that catalyzes the oxidation of bilirubin to biliverdin. Oxygen is the electron acceptor and is reduced to water. The enzyme is used for diagnostic analysis of bilirubin in serum during medical examinations of the serum. It has also been used as an enzymatic catalyst for the cathode of biofuel cells that work under neutral conditions. It is a monomeric protein with a molecular mass of 60 kDa and high reactivity at neutral pH.[8] BOD participates in porphyrin and chlorophyll metabolism.[7]
• Unit Definition: One unit will oxidize 1.0 μmole of bilirubin per min at pH 8.4 at 37 °C.