N9284

Nitroreductase from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli

Manufacturer: Sigma Aldrich

Synonym(S): NTRA

Select a Size

Pack Size SKU Availability Price
1 MG N9284-1-MG In Stock ₹ 39,893.40

N9284 - 1 MG

₹ 39,893.40

In Stock

Quantity

1

Base Price: ₹ 39,893.40

GST (18%): ₹ 7,180.812

Total Price: ₹ 47,074.212

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥100 units/mL

mol wt

monomer 24000

greener alternative product characteristics

Waste PreventionSafer Solvents and AuxiliariesDesign for Energy EfficiencyLearn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

UniProt accession no.

P38489

greener alternative category

, Re-engineered

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Description

  • General description: Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.[1]
  • Application: Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE).[2] It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.[3]
  • Biochem/physiol Actions: Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones.[1] The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.[4]
  • Physical properties: Lyophilized powder containing PBS. Does not contain BSA as excipient
  • Unit Definition: One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
  • Preparation Note: Produced using animal component-free materials.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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expressed in E. coli

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200

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≥90% (SDS-PAGE)

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lyophilized powder

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≥100 units/mL

mol wt:
monomer 24000

greener alternative product characteristics:
Waste PreventionSafer Solvents and AuxiliariesDesign for Energy EfficiencyLearn more about the Principles of Green Chemistry.

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P38489

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