Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns

Description

• Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns
• PepMap LC columns offer lengths targeting fast or high resolution peptide separation
• High resolution in protein identification, biomarker discovery, and systems biology Particular loadability allows highest sensitivity in LC/MS Designed for TFA-free LC-MS, minimizing ion-suppression effects Optimized for coupling to ESI/MS and MALDI-MS Highest column-to-column reproducibility Easy-to-use, cutting-edge miniaturized HPLC Preassembled with nanoViper column inlet fittings for easy column installation Key advantages of nanoViper fittings Ease of use, no sleeve for small capillaries Tool-free assembly No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third-party valves and unions No experimental failure due to bad connections Ideal for high-resolution peptide mapping of tryptic, natural, and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology
• High loading capacity Use for the analysis of low abundant peptides often found in complex proteomics samples Select the column length and I.D
• for your throughput, sensitivity, and amount of sample available requirements Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000μm I.D
• Superior protein and peptide separations Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity Outstanding separation efficiency Superior resolution and recovery Easy installation with nanoViper™ Fingertight Fittings PepMap nanocolumns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar
• Requires no tools Provides virtually zero-dead-volume connections Withstands pressures up to a 1000 bar About Nano, capillary, and micro Acclaim PepMap columns Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses
• Sample amount is often limited and smaller I.D
• columns can increase in sensitivity in sample-limited situations
• While nano LC columns are often used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or for facilitating LC-MS interfacing
• Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and microcolumn in a variety of chemistries for peptides and protein analysis
• Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection
• The trap columns provide the highest efficiencies for one-dimensional peptide mapping experiments and 2D-LC analyses.