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Diameter (Metric)
4 mm
Particle Size
5 μm
Stationary Phase
C18 (octadecyl)
Matrix
C18 (octadecyl) Phase
Phase
Reversed Phase
Length (Metric)
250 mm
Pore Size
100 Å
Product Line
Kromasil
Packing Material
Silica-based Monomeric
Type
HPLC Column
Description
Description Kromasil Classic 100 Å family of products is used to separate and purify molecules of up to about 10,000 Da
Kromasil™ C18 HPLC column involves a silica-based packing
The reduced levels of Na, K, Ca, Fe, Al, and Mg make the column useful for polar compounds, which tend to interact with the silica surface
Reverse phase (RP) HPLC columns are advantageous in ensuring high precision data, they are widely available commercially and are easy to use and less time-consuming
Two main approaches one should consider before choosing a RP column are, firstly characterising physiochemical parameters of the packing material such as 1% carbon load, surface area, pore size, particle size and pH
These are some of the important parameters important to control the quality of the column
Second approach is based upon the chromatographic and statistical evaluation of the retention behaviour for a large number of compounds
Kromasil C18 columns are basically RP columns made up of silica-based monomeric type reversed-phase packing material.