TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™

Description

• High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+ Column is loaded with divalent metal ions by chelation Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at a neutral pH Retained compounds can be eluted with buffer containing imidazole or glycine A decreasing pH gradient can be used for elution purposes as well For successful use of this retention mechanism, select the proper metal ions for chelation and an elution buffer to desorb the analytes Applications: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides