7189939

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size

Ascentis™ Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Manufacturer: Fischer Scientific

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Particle Size

5 μm

Pore Size

90 Å

Product Line

Ascentis

Endcapped

Yes

Technique

Fused-Core HPLC

pH

1 to 9

Stationary Phase

PFP (Pentafluorophenyl)

Matrix

PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform

Phase

Reversed Phase

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Description

  • Ascentis™ Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core™ particle design
  • The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis™ Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines
  • In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions
  • As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes).

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