7189972

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 3 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Manufacturer: Fischer Scientific

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Particle Size

3 μm

Surface Area

100 m2/g

Product Line

Discovery

Endcapped

Yes

Technique

Wide Pore HPLC

Pore Size

300 Å

Stationary Phase

C18 (octadecyl)

Matrix

C18 (octadecyl) Phase/silica particle platform

Phase

Reversed Phase

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Description

  • Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity
  • To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample
  • The more peptides, the better the information
  • Discovery™ BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests
  • The improvements in silica and bonded-phase chemistry that have been incorporated into the Discovery™ BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing
  • An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

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