7190073

MilliporeSigma™ Supelco™ apHera™ C18 HPLC Column, 5 μm Particle Size

apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either.

Manufacturer: Fischer Scientific

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Particle Size

5 μm

Stationary Phase

C18 (octadecyl)

Matrix

C18 (octadecyl) Phase

Pore Size

300 Å

Product Line

apHera

Phase

Reversed Phase

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Description

  • apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either
  • This was accomplished using a vinyl alcohol copolymer base that keeps the surface wetted even with high carbon loads
  • The porous structure has an average pore diameter large enough to produce ideal results for small analytes, peptides and small proteins
  • These columns equal silica based columns in separation efficiency with organic solvents but provide efficiency with buffered alkaline solutions not possible on silica
  • One of the most significant features is logicial elution order of alkylated bases where retention increases proportionately with increasing chain length.Features of apHera™ Reversed Phase Columns
  • Stable vinyl copolymer base
  • Stable pH range 2-12
  • 300 angstrom pore size
  • Stable in all organic solvents
  • Expanded applications: peptides, proteins
  • Efficient separation of any basic substance
  • Amenability to washing and alkaline solutions

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