Invitrogen™ Epi5™ Episomal iPSC Reprogramming Kit

Description

• The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr
• Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University
• This system produces transgene-free, virus-free human induced pluripotent stem cells (iPSCs) with efficiencies in the range of 0.04% to 0.3%, depending upon the cell type being reprogrammed
• Features of the Epi5™ Episomal iPSC Reprogramming Kit include: Easy to use and highly efficient—no need for small molecules during reprogramming and the added p53 suppression provides enhanced iPSC generation (1) Usable for all stages of your iPSC research—transgene-free and viral-free reprogramming allows use from basic to pre-clinical research Flexibility in media systems—can be used in either feeder-free or feeder-based media systems Create Transgene and Virus-free iPSCs Episomal vectors are a well-described system for producing transgene-free, virus-free iPSCs, providing a source of iPSCs for all stages of your pluripotent stem cell research
• Other reprogramming methods, such as lentivirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results
• As oriP/EBNA1 vectors, these episomal vectors contain 5 reprogramming factors (Oct4, Sox2, Lin28, Klf4, and L-Myc) and replicate extra-chromosomally only once per cell cycle
• At this replication rate, the episomes are lost at a rate of approximately 5% per cell generation
• This system shows enhanced iPSC generation through p53 suppression, and the inclusion of L-Myc has been shown to be more potent and specific then c-Myc during human iPSC generation (1)
• Easy-to-Use System This 2-vial kit provides the optimal system for episomal vector reprogramming and eliminates the need for use of small molecules in the reprogramming medium
• The episomal vectors are introduced into the cell by electroporation
• We recommend use of the Neon™Transfection System for electroporation, allowing flexibility in the volume and thus number of cells needed for reprogramming
• Flexibility in Selection of Media Systems The Epi5™ Episomal iPSC Reprogramming Kit can be used with multiple cell types and media systems
• Protocols for fibroblasts and CD34+ blood cells are available in either feeder or feeder-free based reprogramming
• 1
• Okita K, Matsumara Y, Sato Y, et al
• A more efficient method to generate integration free human iPS cells
• Nature Methods 8, 409-412, 2011.