Abnova™ Exosome Standard (A549 cell line)

Description

• Reconstitute lyophilized exosome standard by adding deionized water
• 30 μL or 100 μL for lyophilized standard to get a final concentration of 1 μg/μL
• Different volumes of deionized water for exosomes reconstitution can be chosen by the users in according with the desired final concentration
• Suspend exosomes pipetting the solution up and down 10-15 times and avoid bubbles
• Vortex the reconstituted standard for 60 seconds
• Briefly centrifuge the tubes containing the standard to ensure that the solution is collected at the bottom of the tube
• Pipette the solution up and down 10 times, avoiding the introduction of bubbles
• After this step, the standard is ready to use
• Exosomes are purified following a combination of ultracentrifugation and microfiltration steps
• Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis)
• Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids)
• Lyophilized exosome standards can be stored at 4°C for up to 36 months
• Reconstituted exosome standards are not suitable for long-term storage at room temperature, use them within 2 hours after reconstitution
• The remaining reconstituted standard stock solution should be aliquoted into low binding polypropylene vials and stored at -20°C for up to 1 month or at -80°C for up to 6 months
• Strictly avoid repeated freeze-and-thaw cycles
• Raising in host species: Human Quality control testing: Comparison of exosomal markers on fresh and lyophilized exosomes Type clonality: Protein Applications: Western Blotting, Elisa, Flow Cytometry