89-998-632

Abnova™ Exosome Standard (A549 cell line)

Manufacturer: Abnova™

Select a Size

Pack Size SKU Availability Price
Each of 1 89-998-632-Each-of-1 In Stock ₹ 89,324.64

89-998-632 - Each of 1

₹ 89,324.64

In Stock

Quantity

1

Base Price: ₹ 89,324.64

GST (18%): ₹ 16,078.435

Total Price: ₹ 1,05,403.075

Content And Storage

Store at 4°C for up to 36 months

Description

Storage Buffer: Lyophilized from cell culture supernatant

Lyophilized

Yes

Quantity

2 x 100 μg

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Description

  • Reconstitute lyophilized exosome standard by adding deionized water
  • 30 μL or 100 μL for lyophilized standard to get a final concentration of 1 μg/μL
  • Different volumes of deionized water for exosomes reconstitution can be chosen by the users in according with the desired final concentration
  • Suspend exosomes pipetting the solution up and down 10-15 times and avoid bubbles
  • Vortex the reconstituted standard for 60 seconds
  • Briefly centrifuge the tubes containing the standard to ensure that the solution is collected at the bottom of the tube
  • Pipette the solution up and down 10 times, avoiding the introduction of bubbles
  • After this step, the standard is ready to use
  • Exosomes are purified following a combination of ultracentrifugation and microfiltration steps
  • Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis)
  • Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids)
  • Lyophilized exosome standards can be stored at 4°C for up to 36 months
  • Reconstituted exosome standards are not suitable for long-term storage at room temperature, use them within 2 hours after reconstitution
  • The remaining reconstituted standard stock solution should be aliquoted into low binding polypropylene vials and stored at -20°C for up to 1 month or at -80°C for up to 6 months
  • Strictly avoid repeated freeze-and-thaw cycles
  • Raising in host species: Human Quality control testing: Comparison of exosomal markers on fresh and lyophilized exosomes Type clonality: Protein Applications: Western Blotting, Elisa, Flow Cytometry

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Content And Storage:
Store at 4°C for up to 36 months

Description:
Storage Buffer: Lyophilized from cell culture supernatant

Lyophilized:
Yes

Quantity:
2 x 100 μg

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Content And Storage:
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.

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Quantity:
100 μL

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Abnova Corporation

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Content And Storage:
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.

Description:
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Lyophilized:
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Quantity:
100 μL

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Content And Storage:
Store at 4°C for short term storage. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.

Description:
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Lyophilized:
__

Quantity:
100 μL