Thermo Scientific™ Beta Lysin Disk

Description

• Detect the production of CAMP factor by Group B streptococci with Thermo Scientific™ Remel™ Beta Lysin Disk Christie, Atkins and Munch-Peterson 1 observed a lytic phenomenon when hemolytic and non-hemolytic Group B streptococci are grown in a zone of staphylococcal beta lysin activity
• This phenomenon was used to develop a test that presumptively identified Group B streptococci
• This test was known as CAMP test 2
• A filter paper disk impregnated with beta lysin is used as a substitute for Staphylococcus aureus culture
• Group B streptococci produce a protein-like compound, CAMP factor, which together with beta lysin produces potent hemolysis in sheep blood agar
• Easy to interpret : Positive test- crescent-shaped zone of hemolysis
• Negetive test- no hemolytic zone or small helmet-shaped hemolytic zone
• Ready to use : Reagent-impregnated disk
• Beta Lysin Disk supplies beta lysin obtained from Staphylococcus aureus that acts synergistically with CAMP factor to produce a crescent-shaped zone of complete hemolysis on the sheep blood agar
• Beta lysin reacts with sphingomyelin in the sheep erythrocyte membrane to produce ceramide
• CAMP factor reacts with ceramide to disorganize the erythrocyte lipid bilayer causing an arrowhead or crescent shaped area of complete hemolysis
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.