Thermo Scientific™ Nitrocefin Disk

Description

• Detect β-lactamase production by Neisseria gonorrhoeae , Haemophilus influenzae , Moraxella catarrhalis , Staphylococcus spp., Enteroccoccus spp., and anaerobic bacteria with Thermo Scientific™ Remel™ Nitrocefin Disk
• The β-lactamase enzyme is produced by various organisms and is responsible for their resistance to penicillins and cephalosporins 1,2
• Since then, similar enzymes with somewhat different substrate specificities have been identified in various bacterial species
• The test methods used to detect β-lactamase include iodometric, acidimetric, and chromogenic procedures 3,4
• Since Abraham and Chain confirmed β-lactamase production by microorganisms to be responsible for antibiotic resistance, similar enzymes have been identified in various species
• Some enzymes selectively hydrolyze penicillin class antibiotics (i.e., penicillinases), other enzymes hydrolyze all β-lactamase antibiotics except carbapenems (i.e
• cephalosporinases) and some enzymes hydrolyze both 5
• Ready to use - Nitrocefin impregnated disks
• Rapid results Conforms to CLSI - Performance Standards for Antimicrobial Disk Susceptibility Tests – (Approved Standard M2-A8 and M11-A5) Easy to interpret - With color change The cephalosporin nitrocefin is the substrate used in this test
• β-lactamase hydrolyzes the β-lactam ring of nitrocefin, producing cephalosporanic acid 6
• This results in the pale yellow nitrocefin changing to a pink product upon hydrolysis 7
• Aerobic and anaerobic β-lactamase-producing bacteria affect this color; organisms that fail to produce β-lactamase do not alter the pale-yellow color of nitrocefin within the time limits of test.