Thermo Scientific™ Microdase Disks

Description

• Differentiate Staphylococcus from Micrococcus by detecting the oxidase enzyme with Thermo Scientific™ Remel™ Microdase Disk
• In 1956, Kovacs 1 described the oxidase method that can be used to differentiate Gram-negative bacilli
• Faller and Schleifer 2 modified Kovacs' oxidase reagent by using a tetramethyl-p-phenylenediamine (TMPD) in dimethyl sulfoxide (DMSO)
• Tarrand and Groschel 3 further tested modified Kovacs and confirmed it to be far superior to Kovacs' oxidase reagent
• All micrococci have cytochrome c whereas most staphylococci (with a few exceptions) lack this type of cytochrome
• The oxidase reagent confirms the presence of cytochrome c 4 by exhibiting color change to blue or purple
• Ready to use : Reagent-impregnated disk
• No further preparation required
• Easy to read : Visible color change that confirms positive test Positive test: Blue or purple color development within 2 minutes Negative test: No color change (white to grey color remains) within 2 minutes
• Each Microdase Disk is impregnated with tetramethyl-p-phenylenediamine (TMPD) in dimethyl sulfoxide (DMSO)
• The DMSO provides solubility and stability against auto-oxidation and enhances the permeability of TMPD into the bacterial cell
• In presence of atmospheric oxygen, the oxidase enzyme reacts with TMPD and cytochrome c in the organism to form the colored compound indophenol
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.