Thermo Scientific™ E. coli Screen w/Kovacs

Description

• Presumptively identify Escherechia coli using the Thermo Scientific™ Remel™ E
• coli Screen w/Kovacs
• In 1981, Godsey reported the utility of enzyme activity profiles for rapid identification of Enterobacteriaceae 1
• He observed that E.coli and Shigella sonnei can be differentiated from other species by detecting β-glucuronidase
• Trepeta and Edberg further demonstrated that detecting β-glucuronidase along with oxidase, indole and lactose fermentation can be used for rapid, cost-effective identification E
• coli 2,3
• Escherechia coli is the most commonly found bacteria in clinical laboratory settings involving a wide range of diseases ranging from infected urinary tract to gram negative sepsis 4
• E.coli screen is medium recommended for presumptive identification of E
• coli
• Ready to use : E
• coli screen tubes and Kovac’s Indole Reagent
• Easy interpretation: Visible colour change E
• coli Screen w/Kovacs contains E
• coli Screen Tubes and Kovac’s Indole Reagent
• The E
• coli screen tubes contain Columbia Agar, tryptophane and ρ-nitrophenyl β-D-glucopyranosiduronic acid (PGUA)
• PGUA is a colorless compound that is hydrolyzed by β-glucuronidase to form nitrophenyl, a yellow compound
• In the indole test, tryptophanase enzyme attacks the side chain of tryptophan and leaves an aromatic ring in the form of indole
• This reaction occurs as a condensation process that is formed by an acid splitting of the protein molecule
• The ρ-dimethylaminobenzaldehyde in Kovacs reagent then combines with indole to form a red color complex 5,6
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.