Thermo Scientific™ Escherichia coli O157 Latex Test

Description

• A latex agglutination test for the identification of E
• coli serogroup O157
• Certain strains of Escherichia coli have been implicated in cases of haemorrhagic colitis (HC) and haemolytic ureamic syndrome (HUS)
• It has been shown that these strains produce a vero-cytotoxin (VT)
• The E coli serotype most frequently isolated from HC and HUScases is O157:H7
• Isolation of this serotype from a diarrhoeal stool particularly with the presence of blood is indicative of a vero-cytotoxin producing strain 1, 2, 3, 4, 5, 6, 7 ,8
• The Oxoid E coli O157 latex test will demonstrate by slide agglutination E
• coli strains possessing the O157 serogroup antigen
• The test is best used in conjunction with Sorbitol MacConkey Agar (Oxoid CM0813)
• E
• coli O157:H7 strains do not ferment Sorbitol and therefore give colourless colonies on this medium
• The majority of E coli isolates do ferment Sorbitol and give characteristic pink colonies
• Sorbitol MacConkey Agar should be used as the primary screen
• Non-sorbitol fermenting colonies can then be tested with the latex reagents, to determine whether the isolate belongs to the O157 serogroup and therefore a potential VT- producing strain
• Use this dry latex test designed to demonstrate by slide agglutination E
• coli strains possessing the O157 serogroup antigen
• The test is best used in conjunction with Sorbitol MacConkey Agar
• E
• coli O157:H7 strains do not ferment sorbitol and, therefore, give colorless colonies on this medium
• The majority of E
• coli isolates do ferment sorbitol and give characteristic pink colonies
• Sorbitol MacConkey Agar should be used as the primary screen
• Non-sorbitol fermenting colonies can then be tested with the latex reagents to determine whether the isolate belongs to the O157 serogroup and, therefore, a potential verotoxin-producing strain
• Pre-dispensed reagents eliminate extra steps for faster time to result Room temperature storage for up to two years simplifies inventory Blue latex particles are sensitized with reactive (positive) and non-reactive (negative) antibodies and dried onto reaction cards for convenience Not all products are available for sale in all territories
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand, combining powerful manual, semi-automated and fully automated test products and a comprehensive line of media and diagnostic products to offer a complete, end-to-end solution to quickly deliver the products you need and the quality results your laboratory depends on.