Thermo Scientific™ Remel™ PYR Reagent

Description

• Rapidly identify Group A Streptococci and Enterococci using Thermo Scientific™ Remel™ PYR Reagent.In 1981, Godsey, et al differentiated group A streptococci and enterococci based on their ability to cleave L-pyrrolidonyl-β-naphthylamide (PYR)1
• Facklam et al
• incorporated PYR substrate into agar and tested for enzyme activity after overnight incubation2
• Bosely et al
• incorporated PYR substrate into broth and tested for enzyme hydrolysis after 4 hours incubation3
• Ellner et al
• described a colorimetric method for the PYR test using filter paper strips containing PYR substrate4
• Use PYR Reagent for rapid identification of Group A Streptococci and Enterococci.Rapid Test - Color change observed within 1-2 minutes after addition of reagentReady-to-use - Liquid reagentEasy to interpret -Red color developmentL-pyrrolidonyl-β-naphthylamide (PYR) serves as the substrate for the detection of pyrrolidonyl peptidase
• The peptidase enzyme hydrolyzes the substrate to beta naphthylamine which produces a red color on addition of N,N-dimethyl-aminocinnamaldehyde (PYR reagent)
• The ease of performance and interpretation of the PYR test suggests that it is more specific than the routine bacitracin test for group A Streptococci and is at least as specific as the routine 6.5% NaCl test for Enterococci5.This reagent contains 0.15 g/1000mL of N,N-dimethyl-aminocinnamaldehyde (PYR reagent).Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.