Thermo Scientific™ Remel™ Anaerobic Nitrate Reagent A

Description

• Detect indole production and nitrate reduction in anaerobic bacteria using Thermo Scientific™ Remel™ Anaerobic Nitrate Reagent A
• In 1977, Wideman et al
• utilized a Nitrate Disk to determine nitrate reductase production and found 89% test agreement with the more conventional Indole Nitrate Medium1
• The microbial processes of denitrification and dissimilatory nitrate reduction to ammonium are two important nitrate reducing mechanisms in soil
• Anaerobes found in soil and sediments are involved in these processes
• Several methods are available to detect nitrate reduction2
• Indole nitrate medium is an aid in the identification of a wide variety of microorganisms.Ready to use:Convenience of a prepared reagent.Ease of Differentiation: Positive test indicates red color development after addition of reagents; and no color development after addition of zinc dust
• Negative test indicates no color development after addition of reagents and red color development after addition of zinc dust.Rapid Results: Within 10 minutes.Dual Purpose: Developed to serve the dual purpose of detecting indole production and nitrate reduction.Certain bacteria convert nitrate to nitrite, ammonia, or gas
• This process is denitrification
• Presence of nitrite is determined by the addition of sulfanilic acid which reacts with the nitrite to form a diazonium salt
• 5-amino-2-naphthalene sulfonic acid then couples with the salt, resulting in a red dye complex
• However, if the organism converted nitrite to ammonia or nitrogen gas, no nitrate remains to react with the sulfanilic acid; therefore, zinc dust must be added
• Zinc reduces nitrate to nitrite, resulting in a red color
• The red color indicates that nitrate is still present and was not previously reduced
• An absence of red color after the addition of zinc dust indicates that no nitrate was reduced further than nitrite3.Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.