Thermo Scientific™ Remel™ 10% Bile Solubility Reagent

Description

• Identify and differentiate Streptococcus pneumoniae from other alpha-hemolytic streptococci with Thermo Scientific™ Remel™ 10% Bile Solubility Reagent.In 1900 Neufeld used sodium desoxycholate to detect autolysis which can be used to differentiate bile-soluble S
• pneumoniae from bile-insoluble, alpha-hemolytic streptococci1
• Downie et al
• used 10% bile salt solution to lyse broth cultures of pneumococci2
• The 10% reagent can provide more rapid reactions and has been reported to provide reliable results with the tube as well as the spot test methods3-5
• Ready to use: liquid reagent.Rapid Test: results in 30 minutes (Spot test) to 3 hours (Tube test).Easy to interpret:Tube test: Positive test: test suspension clears within 3 hours; control suspension remains turbid.Negative test: test and control suspension remain turbid.Spot test:Positive test: colony disintegrates or dissolves within 30 minutes.Negative test: colony remains intact.S
• pneumoniae possess an autolytic enzyme that lyses the organism's own cell wall during cell division
• This autolytic intracellular enzyme causes the organism to undergo rapid autolysis when cultivated on artificial medium6
• The bile solubility test is based on the observation that S
• pneumoniae cells visibly lyse when 10% sodium desoxycholate is applied, while other alpha-hemolytic streptococci do not
• The mechanism for bile solubility is believed to result from bile salts lowering the surface tension at the medium-membrane interface, making it susceptible to disruption by certain chemical agents.10% Bile Solubility Reagent contains 10% w/v Sodium Desoxycholate, Tincture Thimerosal, Ethyl Alcohol and Dimineralized water.Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.