Thermo Scientific™ Remel™ Nitrate Reagent B For AFB

Description

• Differentially identify Mycobacterium spp
• with their ability to utilize nitrate using Thermo Scientific™ Remel™ Nitrate Reagent B For AFB
• Virtanen found that mycobacteria differ in their ability to reduce nitrate by means of nitrate reductase based on the age of the culture, temperature, enzyme inhibitors, and hydrogen ion concentration1
• The nitrate reduction procedure is recommended by Kent and Kubica in A Guide for the Level III Laboratory2
• Mycobacteria, being a slow growing bacteria, needs a faster detection in the specimen samples for rapid diagnosis and treatment of Tuberculosis
• Nitrate reduction is the most sensitive test used for detecting the ability of Mycobacteria to reduce nitrate
• The presence of the enzyme nitrate reductase is an important differential characteristic in identifying different species of Mycobacteria.Ready to Use: Convenience of a prepared reagent.Ease of Differentiation: Positive test indicates red color development after addition of reagents; and no color development after addition of zinc dust
• Negative test indicates no color development after addition of reagents and red color development after addition of zinc dust.Rapid Detection: within 30-60 seconds.The end products of nitrate reduction are many and depend upon the bacterial species
• The most important end product is molecular nitrogen by way of nitrite reduction which is indicated by the presence of a catabolic end product or the absence of nitrate in the medium
• The development of a red color after the addition of three reagents: hydrochloric acid; sulphanilic acid and N-naphthylethylenediamine indicates that the nitrate has bene reduced to nitrite
• The red color reaction is due to the formation of a diazonium compound
• When confirming a negative test with zinc dust, the reduction of the diazonium salt by zinc in the presence of acetic acid produces a colored compound, arylhydrazine.Used in conjunction with Nitrate Reagent A for AFB, Part No
• R21243
• For use with Nitrate Substrate Broth, Part No
• R0615 Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.