Thermo Scientific™ Remel™ Kovacs Indole Reagent

Description

• Determine the ability of an organism to split indole from tryptophan molecule with Thermo Scientific™ Remel™ Kovac's Indole Reagent.Previously xylene was used to detect small amounts of indole produced from breakdown of tryptophan
• Kovac's used amyl alcohol in place of ethyl alcohol to bypass extraction procedure involved in the Ehrlich-Boehme reagent1
• Gadebusch and Gabriel reported that substitution of isoamyl alcohol resulted in greater stability of the Kovacs' reagent2
• Kovac’s Indole Reagent is used to determine the ability of microorganisms, primarily Enterobacteriaceae, to split indole from the tryptophan molecule
• Intracellular enzymes collectively called tryptophanases help in the production of indole by hydrolytic activity against the amino acid tryptophan
• Indole combines with dimethylaminobenzaldehyde to form a red compound
• This is a condensation reaction3.Ready for use: No further preparation is required.Easy to interpret: A pink-red ring between broth and solvent iindicates a positive testKovac’s Indole Reagent contains amyl alcohol (CAS 71-41-0), p-dimethylaminobenzaldehyde (CAS 100-10-7), hydrochloric acid (concentrated) (CAS 7647-01-0)
• This reagent has been tested using quality control procedures and found to be acceptable
• This product is for in vitro diagnostic use and should be used by properly trained individuals.Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.