Thermo Scientific™ Remel™ Ninhydrin Reagent

Description

• Detect hydrolysis of sodium hippurate by beta-hemolytic streptococci, Gardnerella vaginalis, and Campylobacter jejuni with Thermo Scientific™ Remel™ Ninhydrin Reagent.The hippurate hydrolysis test determines the ability of group B streptococci as well as other bacteria to enzymatically hydrolyze sodium hippurate1-3
• Hwang and Ederer employed the Ninhydrin Reagent in a rapid method to detect hippurate hydrolysis by the end product glycine4
• Ninhydrin Reagent is used to detect sodium hippurate hydrolysis by beta-hemolytic streptococci, G
• vaginalis, and C
• jejuni.Hippuric acid is hydrolyzed by the enzyme hippuricase to glycine and benzoic acid
• Ninhydrin evokes a five-step reaction to form hydrindantin (a reduced form of ninhydrin), carbon dioxide, and ammonia
• A condensation reaction happens with hydrindantin, ammonia and residual ninhydrin to produce a final purple colored complex3.Ready for use: no further preparation is required.Easy to interpret:Positive test: Blue to purple color development within 30 minutes.Negative test: Light grey color development or no color change within 30 minutes.Ninhydrin Reagent contains ninhydrin (CAS 485-47-2), acetone (CAS 67-64-1), butanol (CAS 71-36-3) supplied in 25 mL bottles
• This product is for in vitro diagnostic use and should be used by properly trained individuals
• This reagent have been tested using quality control procedure and found to be acceptable.For use with Hippurate Disk, REF R2108Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.