Thermo Scientific™ Remel™ Nitrate Reagent B

Description

• Determine the ability of an organism to reduce nitrate to nitrite or free nitrogen gas using Thermo Scientific™ Remel™ Nitrate Reagent B
• This ability is characteristic of the Enterobacteriaceae1 family
• Nonfermenters and other miscellaneous gram-negative bacilli vary in their ability to reduce nitrates, with some being capable of further reducing nitrite to nitrogen gas2
• In 1962, Daubner reported that all members of the Enterobacteriaceae tested, except for Erwinia species, reducing nitrate in eight hours3
• Determine the ability of an organism to reduce nitrate to nitrite or free nitrogen gas using Nitrate Reagent B.Ready to Use: Convenience of a prepared reagent.Ease of Differentiation: Positive test indicates red color development after addition of reagents; and no color development after addition of zinc dust
• Negative test indicates no color development after addition of reagents and red color development after addition of zinc dust.Rapid Detection: The test can be detected within 10-15 minutes.Nitrate serves as a source of nitrogen for many bacteria and fungi, but to be utilized it must be broken down.The nitrite reduction test is a two-step procedure
• Sulphanilic acid is added to acidify the medium and then naphthylamine is added which reacts with the nitrites to produce a red color
• Certain bacteria convert nitrate to nitrite, ammonia, or gas
• This process is denitrification
• If no color develops after addition of reagents, the organism has either reduced the nitrate to free nitrogen or it has not attacked the nitrate at all
• Nitrate will react with zinc dust to form nitrite
• A red color development after addition of zinc dust indicates that the nitrates are present, indicating a negative nitrite reduction test
• No formation of a red color after addition of zinc dust indicates that the organism has reduced the nitrate to free nitrogen or ammonia and is considered a positive nitrate test.Nitrate Reagent B is to be used in conjunction with Nitrate Reagent A, REF R21239.Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.