Thermo Scientific™ Nitrate Disk

Description

• Determine nitrate reduction by anaerobic bacteria using Thermo Scientific™ Remel™ Nitrate Disks
• In 1977, Widemann et al
• utilized a nitrate disk for the detection of nitrate reductase production 1
• They reported it to be 89% comparable to the more conventional and time-consuming indole nitrate medium assay
• Filter paper disks impregnated with potassium nitrate are recommended for presumptive testing of anaerobes 2,3
• Determine nitrate reduction by anaerobic bacteria using Nitrate Disks
• Ready to use: Reagent-impregnated disk
• Rapid test: Results are obtained within 3-5 minutes
• Easy to identify: A positive reaction showcases a development of red color after addition of Anaerobic Nitrate Reagent A and B and no color change after addition of zinc dust while in a negative reaction there is no color change post addition of reagent but changes to red after addition of zinc dust
• Organisms that possess nitrate reductase can reduce nitrate to nitrite
• Nitrate serves as a source of nitrogen for many bacteria
• Molybdate is reported to stimulate synthesis of nitrate reductase 4
• The first step to nitrate utilization is reduction to nitrite by the removal of one oxygen molecule
• Nitrite combines with sulfanilic acid and amino-2-naphthaline to form a red colored end product
• Certain organisms can further reduce nitrite to nitrogen, yielding a colorless result
• An additional test for the presence of unreacted nitrate must be performed to validate the colorless result
• Zinc dust catalyzes the reaction of nitrate to nitrite
• Therefore, when zinc dust is added, a red color indicates the presence of unreacted nitrate
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.